RBS

Part:BBa_M50080

Designed by: Kara Helmke Rogers   Group: Stanford BIOE44 - S11   (2017-10-17)


Strong RBS

Strong RBS from DNA2.0.

A ribosome binding site (RBS) is a sequence on mRNA that is bound by the ribosome during protein translation. It can be either the 5' cap of a mRNA in eukaryotes, a region 6-7 nucleotides upstream of the start codon AUG in prokaryotes (called the Shine-Dalgarno sequence), or an internal ribosome entry site (IRES) in viruses. Prokaryotic ribosomes recognize RBSs primarily via base-pairing between the RBS and an unstructured end of the 16s rRNA molecule that forms part of the ribosome. Translation initiation rate of a particular mRNA can be regulated by sequence of the RBS, leading to varying strengths - strong, medium or weak. (www.msb.embopress.org/content/7/1/481.abstract)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None